Webaptamer-based quantum dot conjugates for molecular imaging where the conjugation of the aptamers to the quantum dot is facilitated using biotin-avidin interactions ... using the Quant-iT OliGreen ssDNA assay kit from Invitrogen. The gold nanoparticles were frst dissolved in … WebApr 6, 2024 · DNA (ssDNA) from purified libraries using the Quant-iT™ OliGreen® ssDNA Assay Kit (Life Technologies) read on a Molecular Devices Multimode SpectraMax M2 plate Reader. Libraries yielding less than 545 ng of sequencing library are considered as failed. D. Hybrid Capture .
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WebQuant It Oligreen, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebMar 17, 2024 · This assay quantifies ssDNA initial sample concentrations of 50 pg/µL – 200 ng/µL when using 1 – 20 µL of sample in 200 µL assay volume. The kit has a mix and measure protocol and includes buffer, reagent and two standards. Once prepared, it is strongly recommended to measure within 15 minutes for best results. cards for a loss loved one
Quant-iT™ PicoGreen ® dsDNA Reagent and Kits - Thermo Fisher …
WebQuantitation Kit 1 plate R32700 Quant-iT ™ OliGreen ™ ssDNA Assay Kit 1 mL assay kit O11492 Quant-iT ™ OliGreen ™ ssDNA Assay Reagent 1 mL reagent O7582. Table 3 … WebNov 15, 2024 · For quantification and estimation of amplification efficiency, a standard curve was created using increasing concentrations of cDNA. The amplification transcripts were quantified with the relative standard curve and normalized to the concentration of cDNA in each RT sample measured using the Quant-iTTM 338 OliGreen ssDNA Assay Kit … WebFor 20–2000 samples: use the Quant-iT OliGreen ssDNA Assay Kit with microplate reader; Notes: 1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit … brookdale assisted living memory care