Phospho stat3 flow cytometry
WebA specific band was detected for Phospho-STAT3 (Y705) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Intracellular Staining by Flow Cytometry View Larger Detection of Phospho-STAT3 (Y705) in IFN alpha-treated Daudi Human Cell Line by Flow Cytometry. WebClone 49/p-Stat3. Analysis of Stat3 (pS727) in monocytes. Human peripheral blood mononuclear cells (PBMC) were either stimulated with 40 nM PMA (Sigma, P8139) for 15 …
Phospho stat3 flow cytometry
Did you know?
WebSep 1, 2016 · The following phospho-specific monoclonal antibodies were used in 3 different panels during the flow cytometry protocol: Alexa Fluor ®647 conjugated anti-Stat4 … WebThe cells were then stained with BD Phosflow™ BV421 Mouse Anti-Stat3 (pS727) antibody (Cat. No. 565416). The fluorescence histograms showing Stat3 (pS727) expression were derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow ...
WebFlow Cytometry CHARACTERIZATION This antibody is tested by direct flow cytometric analysis of human cells. WORKING DILUTIONS ... (green), using Phospho-Stat3 (Tyr705) (D3A7) XP Alexa Fluor 488 conjugate compared to nonspecific control antibody (red), and flow cytometric analysis of Jurkat cells, untreated (blue) or IFN-α treated (green), using ... WebFlow cytometry can be used to study STAT phosphorylation on a per cell basis. Cells are fixed, permeablized, and stained with antibodies that specifically bind to the …
WebFeb 9, 2011 · Optimize your flow cytometry workflow to attain deeper insights with high-dimensional biology. For a limited time, take advantage of packages that include flow cytometers, reagents and informatics combined with single-cell multiomics instrumentation. Contact Us Innovative solutions to power your diagnostics and research WebOct 3, 2024 · Flow cytometry analysis was performed on BD FACSCanto II cytometer. Data analysis was performed with FCS Express 6 software. The target cells were gated on FSC/SSC plot to remove debris, followed by a singlet gate on FSC-H/FSCW plot. ... SKBr3 cancer cell lines probed for total and phospho-STAT3 by Western blotting. (B) …
WebPhospho Targets Need a control for your phospho flow cytometry staining? Our Veri-Cells™ Phospho PBMCs can be used as a positive and negative control for staining members of …
WebNov 15, 2013 · In order to validate a phospho-specific flow cytometry assay assessing phospho-signal transducer and activator of transcription factor 5 (p-STAT5) as a new diagnostic tool for JMML, we examined 22 samples from children with JMML and 47 controls. CD33+/CD34+ cells from 22 patients with JMML showed hyperphosphorylation … johnstone butchers annanWebDec 21, 2012 · Flow Cytometry. BTSC spheres were dissociated to single cells, ... Millipore), phospho-STAT3 Y705 (1:50; Cell Signaling Technology), phospho-STAT3 S727 (1:50; Cell Signaling Technology), human nucleolin (1:1000; Millipore), and human nuclear antigen (1:20; Chemicon). Staining was visualized with Vectastain Elite mouse immunoglobulin … johnstone butchers falkirkWebSTAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the … how to go out on stress leave and get paidWebDec 1, 2024 · In summary, STAT3 phosphorylation at tyrosine 705 and serine 727 differentially regulates the EMT–MET switch within the distinct molecular subtypes of CSCs to complete the metastatic process. john stoneburner md torrance caWebImmunogen: Phosphorylated Human Stat3 Peptide Application: Intracellular staining (flow cytometry) (Routinely Tested) Vol. Per Test: 20 µl RRID: AB_1645327 Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide. Regulatory Status: RUO View All Formats Preparation And Storage how to go over thick part of jeans hemWebDec 1, 2024 · In summary, STAT3 phosphorylation at tyrosine 705 and serine 727 differentially regulates the EMT–MET switch within the distinct molecular subtypes of CSCs to complete the metastatic process. johnstone butchers bedfordWebPhospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb (PE Conjugate) detects endogenous levels of Stat3 only when phosphorylated at Tyr705. This antibody does not cross-react … how to go pageless in google docs