TīmeklisLambda DNA, 250ug NEB #N3011S $58 Klenow Fragment (3' ->5' exo -), 200 units NEB #M0212S $56 Quick Ligation Kit, 30 reactions NEB #M2200S $95 Taq DNA Polymerase with Standard Taq Buffer , 400 units NEB #M0273S $58 Antartic Phosphatase , 1,000 units , 5,000 units/mL NEB #M0289S $58 QIAquick PCR ... Tīmeklis< 15 kb, enrichment will be less efficient. Determine DNA quality and quantity by agarose gel electrophoresis of the sample alongside a DNA marker (e.g., Lambda DNA-HindIII Digest, NEB #N3012). It is also important to quantitate the amount of DNA in the experimental sample by A

Lambda Exonuclease (M0262) - New England Biolabs Japan

TīmeklisThermo Scientific Lambda is a temperate Escherichia coli bacteriophage. The virion DNA is linear and double-stranded (48502 bp) with 12 bp single-stranded … TīmeklisLambda DNA. Lambda DNA, a linear, double-stranded phage DNA containing 12 bp single-stranded complementary 5'-ends, is derived from an Escherichia coli bacteriophage (Bacteriophage lambda cI 857 Sam7). Lambda DNA (48,502 bp) may be used as a molecular weight size marker during nucleic acid gel analysis following … navien remote control wire

New England Biolabs Certificate of Analysis

TīmeklisLambda DNA (dam-) is 48,502 base pairs in length. Source The phage is isolated from a heat-inducible E. coli(dam-) lambda lysogen. The DNA is isolated from purified … Tīmeklis2024. gada 16. jūl. · Taq DNA ligase (NEB, M0208L) was used to ligate the oligonucleotide on Lambda, using 10 times molar access of oligonucleotide to Lambda DNA. The mixture was incubated for 10 [ at 65°C and then 1hour at 50°C in Taq DNA ligase buffer. The biotin-Lambda DNA was then cleaned up from free oligonucleotides TīmeklisQuick-Load® DNA Marker, Broad Range (NEB #N0303) 200/400 µl (100 µg/ml) The Quick-Load DNA Marker, Broad Range is a mix of HindIII digest of Lambda DNA … market mastery morpheus