WebJun 12, 2007 · I am analysing drug in capsule (gelatin capsule) and getting a huge hump before the parent peak. The method works fine for the drug substance. the hump is coming where the two impurities are supposed to be. My mobile phase A is 5/95/0.1:ACN/H2O/TFA, mobile phase B is 95/5/0/1:ACN/H2O/TFA. The Column is C18 and the gradient is Initial-- … WebColumn collapse of analysis column or protective column. This is a common situation. Change the analysis column or guard column, then compare the peak shape. Peak …
Ghost peaks in reversed-phase gradient HPLC: a review and update
WebPoor fitting need to be investigated from peak tailing. Make sure that the connections are tight but overtighten fittings will permanently damage the fitting and increase in system … WebSep 5, 2024 · 3.2: High Performance Liquid chromatography. High-performance liquid chromatography (HPLC) is a technique in analytical chemistry used to separate the components in a mixture, and to identify and quantify each component. It was initially discovered as an analytical technique in the early twentieth century and was first used to … remington ltr 308
HPLC: What to do in case of peaks being too broad?
WebThe “hump” typically shows as a broad peak, indicating “something” is eluting form the column. Normally the base line should be stable after the elution of the peak marked “X”. … WebSep 9, 2024 · HAWACH introduces some common peak shapes and possible causes here and we will see whether there is a relationship with the HPLC column. 1. No peak. … WebHPLC Troubleshooting Guide 1 HPLC Troubleshooting Uwe D. Neue, Waters Corporation 1. Column Life-Time 2. Variable Retention Times 3. Drifting Retention Times 4. Column-to-Column and Batch-to-Batch Reproducibility 5. Sample Preparation Problems 6. Sources of Peak Tailing 7. Normal-Phase Chromatography 8. System Volume, Dead … profil bluetooth mains libres