NettetCell cycle analysis by quantitation of DNA content was one of the earliest applications of flow cytometry. The DNA of mammalian, yeast, plant or bacterial cells can be stained by a variety of DNA binding dyes. The premise of these dyes is that they are stoichiometric, i.e. they bind in proportion to the amount of DNA present in the cell. Nettetfluorescence based assays, Hoechst 33342 stock solutions should be diluted in phosphate-buffered saline or mounting medium to a final working concentration of 1 …

Hoechst 33342 for 72h live imaging, is it ok - ResearchGate

NettetAdd 10 × PBS to a final concentration of 1 × PBS (e.g. 100 mL 10 × PBS in 900 mL 4 % PFA/dH 2 O). Adjust pH to 7.2–7.4 with HCl. Methanol (prechilled to –20 °C): ... Nettet9. nov. 2024 · Tip: Quench excess formaldehyde with 10 mM ethanolamine in PBS (or 0.1 M glycine in PBS) for 5 min. Tip: Add 0.1% Triton X-100 in PBS into the fixed cells for 3–5 minutes to increase permeability. Then wash cells 2–3 times in PBS. Tip: If cells do not appear healthy, add serum (2–10% range) to stain and wash solutions. ds gbaスロット

Arg-tRNA synthetase links inflammatory metabolism to RNA …

Nettet1) Stock H342 solution: H342 should be originally suspended in dH2O at a 1mM concentration (H342 will precipitate in PBS). 2) Cell suspension: This procedure is very sensitive to cell concentration and pH of the media. Cells should be approximately 1-2×106/ml, in buffered media, pH 7.2. NettetDilute Hoechst to the desired concentration in PBS or H2O. Remove media from the cells and rinse once with PBS. Add the diluted Hoechst and then incubate for 15-30 minutes protected from... NettetThe effect attained was similar at all three concentrations, but there was a problem while conducting an image acquisition. All the analogs were stained well with hoechst at 1ug/ml except in... dsgetdcname 呼び出しが失敗しました。エラー 1355